This method is for the determination of polynuclear aromatic hydrocarbons (PAH) from municipal and industrial wastewater. One liter of sample is extracted with methylene chloride using a separatory funnel. The extract is dried and concentrated to a volume of 10 mL or less and analyzed using high performance liquid chromatography with a ultraviolet detector (HPLC/UV) and gas chromatography with a flame ionization detector (GC/FID).
The GC/FID procedure does not adequately resolve the following four pairs of compounds: anthracene and phenanthrene; chrysene and benzo(a)anthracene; benzo(b)fluoranthene and benzo(k)fluoranthene; and dibenzo(a,h) anthracene and indeno (1,2,3-cd)pyrene. The HPLC/UV method does resolve all PAHs indicated in the method.
A silica gel column cleanup can be used if there are matrix interferences in the sample.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | 7 days from sample collection to extraction and 40 days from extraction to analysis. |
| Preservatives: | Cool to 4°C and protect from light. If residual chlorine is present add 80mg of sodium thiosulfate per Liter of sample. |
| Required Preps: | 1L amber glass bottle |
| Collection Method: | Grab sampling |
| Analytical Methodology: | GC/FID or HPLC/UV |
| Documentation: | 
610
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| Anthracene | C14H10 |
120-12-7 |
0.66 |
µg/L |
| Pyrene | C16H10 |
129-00-0 |
0.27 |
µg/L |
| Benzo(g,h,i)perylene | C22H12 |
191-24-2 |
0.076 |
µg/L |
| Indeno(1,2,3-cd)pyrene | C22H12 |
193-39-5 |
0.043 |
µg/L |
| Benzo[b]fluoranthene | C20H12 |
205-99-2 |
0.018 |
µg/L |
| Fluoranthene | C16H10 |
206-44-0 |
0.21 |
µg/L |
| Benzo[k]fluoranthene | C20H12 |
207-08-9 |
0.017 |
µg/L |
| Acenaphthylene | C12H8 |
208-96-8 |
2.3 |
µg/L |
| Chrysene | C18H12 |
218-01-9 |
0.15 |
µg/L |
| Benzo[a]pyrene | C20H12 |
50-32-8 |
0.023 |
µg/L |
| Dibenz[a,h]anthracene | C22H14 |
53-70-3 |
0.03 |
µg/L |
| Benzo(a)anthracene | C18H12 |
56-55-3 |
0.013 |
µg/L |
| Acenaphthene | C12H10 |
83-32-9 |
1.8 |
µg/L |
| Phenanthrene | C14H10 |
85-01-8 |
0.64 |
µg/L |
| Fluorene | C13H10 |
86-73-7 |
0.2 |
µg/L |
| Naphthalene | C10H8 |
91-20-3 |
1.8 |
µg/L |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.