This method is for the determination of (2,4-dichlorophenoxy)acetic acid (2,4-D), and (2,4,5-trichlorophenoxy)acetic acid (2,4,5-T). The sample is collected on a binderless glass fiber filter and desorbed in 15 mL of methanol. The sample is analyzed by HPLC with a UV detector. This method is applicable for 2,4-D, 2,4,5-T, and their salts, but not their esters. The working range of the method is 1.5 to 20 mg/m³ of either compound for a 100-L air sample. Note: This method combines and replaces Methods S279 and S303 which are the same except for eluent composition and UV detector wavelength.
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| Hold Times, Preservatives, Preps, Collection, Analytical & Documentation | |
|---|---|
| Holding Time: | The method states the samples are stable for at least 1 week if kept at 25°C. |
| Preservatives: | Keep samples at 25°C. |
| Required Preps: | 37 mm Binderless glass fiber filter |
| Collection Method: | The method recommends a 1 - 3L/min. flow rate with a 15L to 200L sample collection volume. |
| Analytical Methodology: | HPLC/UV |
| Documentation: | 
5001
|
| Analyte | Formula | CAS Number | Detection Limit | |
|---|---|---|---|---|
| 2,4,5-T | C8H5Cl3O3 |
93-76-5 |
0.015 |
mg |
| 2,4-D | C8H6Cl2O3 |
94-75-7 |
0.015 |
mg |
* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.