Method 64

Glutaraldehyde by HPLC/UV

This method describes the sample collection and analysis of glutaraldehyde. An air sample is collected by drawing a known volume of air through an open-face air monitoring cassette containing 2 glass fiber filters, each of which is coated with 2,4-dinitrophenylhydrazine and phosphoric acid. The sample filters are extracted separately with acetonitrile and analyzed by HPLC using a UV detector.



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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   The recovery of glutaraldehyde from samples used in a 17-day storage test was essentially 100% when the samples were stored at about 23°C, or frozen at -20°C.
Preservatives:   Keep refrigerated or at ambient temperature in the dark.
Required Preps:   Open-face air monitoring cassette containing 2 glass-fiber filters which are separated and retained using cassette rings. Each filter is coated with DNPH and phosphoric acid.
Collection Method:   Glass fiber filters: 15L @ 1L/min.
Analytical Methodology:   HPLC-UV.
Documentation:   064

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.