Method 613

2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD)

This method is for the determination of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in municipal and industrial wastewater.  One liter of sample is spiked with a carbon 13 labeled 2378-TCDD internal standard and extracted with methylene chloride using a separatory funnel.  The extract is solvent exchanged to hexane, concentrated and analyzed by GC/MS (gas chromatography/mass spectrometry).  This is an isotope dilution method.

The method specifies alumina column and silica gel cleanups that may be used if there are interferences found in the sample matrix.

(EPA: Office of Water)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   7 days from sample collection to extraction and 40 days from extraction to analysis.
Preservatives:   Cool samples to 4°C and protect from light. If residual chlorine is present, add 80 mg of sodium thiosulfate per Liter of sample.
Required Preps:   1L amber glass bottle
Collection Method:   Grab sampling
Analytical Methodology:   GC/MS
Documentation:   613

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.