Method 2532

Glutaraldehyde by HPLC-UV

This method is for the determination of glutaraldehyde. The sample is collected on silica gel coated with 2,4-DNPH HCl sorbent tubes, desorbed in acetonitrile and shaken for 2 hours, followed by HPLC-UV analysis. The working range is 0.01 to 0.3 ppm (0.04 to 1.2 mg/m³) for a 20 L air sample. NOTE: OSHA method 64 uses a glass fiber coated with 2,4-DNPH and phosphoric acid for collection, with HPLC analysis.

(NIOSH Issue 1: 15 August 1994)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   At least 30 days @ 25 °C
Preservatives:   25 °C
Required Preps:   Solid sorbent tube: silica coated with 2,4-dinitrophenyl hydrazine HCl, 300mg/150mg; 110mm x 6mm OD.
Collection Method:   Solid sorbent tube, 1L to 30L @ 0.05 to 0.5 L/min
Analytical Methodology:   HPLC-UV
Documentation:   2532

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.