Method 3514


This method is for the determination of ethylenimine. The sample is collected through a bubbler containing Folin's reagent, extracted twice with chloroform and then analyzed by HPLC with ultraviolet detection. The working range is 0.014 to 11 ppm (0.025 to 20 mg/m³) for a 40-L air sample. A variation of this method has been used to measure ethylenimine in air at 0.02 to 0.10 mg/m³ at a manufacturing plant. Note: This method replaces Method P&CAM 300.

(NIOSH Issue 2: 15 August 1994)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   14 days @ 5 °C in dark.
Preservatives:   Ship and store at 5 °C in dark.
Required Preps:   Glass bubbler containing Folin's reagent.
Collection Method:   Glass bubbler: 1L - 48L @ 0.2L/min.
Analytical Methodology:   HPLC/UV.
Documentation:   3514

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.