Method 5509

Benzidine and 3,3'-Dichlorobenzidine by HPLC-UV

This method is used for the determination of benzidine and 3,3'-dichlorobenzidine. The sample is collected on a glass filter, desorbed with 0.17% (v/v) triethylaminine in methanol, and then analyzed by HPLC and UV detection. The working range is 4 to 200 mg/m³ for a 50 L air sample. NOTE: This combines and replaces P&CAM 243 and P&CAM 246.

(NIOSH Issue 2: 15 August 1994)


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Method Data

Hold Times, Preservatives, Preps, Collection, Analytical & Documentation
Holding Time:   12 days at 23 °C (11 days at -15 °C for benzidine)
Preservatives:   Dry Ice
Required Preps:   13-mm Glass fiber filter
Collection Method:   Filter: 20 L- 100 L @ 0.2 L/min.
Analytical Methodology:   HPLU, UV detection
Documentation:   5509

Analyte List*

Analyte Formula CAS Number Detection Limit

* The analytes and detection limits listed for each method represent the typical detection limits and analytes reported for that particular method. Keep in mind that analyte lists may vary from laboratory to laboratory. Detection limits may also vary from lab to lab and are dependent upon the sample size, matrix, and any interferences that may be present in the sample.